How to perform a CRISPR Knockout Experiment

Опубликовано: 21 Март 2017
на канале: Applied Biological Materials - abm

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Due to CRISPR's unparalleled ease-of-use and affordability, gene knockout experiments are now more feasible than ever before!

In this video, we will show you how we used the CRISPR Cas9 system to knockout the LIF gene in a colon carcinoma cell line. This is accomplished by the delivery of a Cas9 and sgRNA lentivector into the cell line followed by drug selection and monoclonal isolation. More importantly, we demonstrate how to characterize and verify your CRISPR Knockout using the PCR-based Surveyor Assay, Sanger Sequencing, and Next Generation Sequencing. This complete process of validation ensures that your final CRISPR Knockout cell line is 100% successful.

At the end of the video, we will introduce you to abm’s entire suite of CRISPR Knockout services – designed to help you jumpstart your Knockout experiments!

Read our complete CRISPR Knockout Case Study here:
➜ https://info.abmgood.com/crispr-cas9-...

Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse: http://info.abmgood.com/crispr-crash-...

Read more about the basics of the CRISPR system on our knowledge base:
➜ https://info.abmgood.com/crispr-cas9

Learn more about our CRISPR Knockout Cell Line Generation Service here:
➜ https://goo.gl/EpEZDB

Watch more videos about CRISPR:
➜CRISPR Cas9 - Gene Regulation with dCas9: https://goo.gl/tRkHWd
➜ How a mini CRISPR Cas9 system is revolutionizing gene therapy! https://goo.gl/njRPv2

Thank you to all of the amazing community contributions towards translating this video into other languages including:
➜ Spanish
(To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.)

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