Sanger DNA Sequencing, From Then to Now.

Опубликовано: 30 Май 2023
на канале: ClevaLab

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This video explores the basics of Sanger sequencing and the fascinating history behind this groundbreaking technology. It explains the basics of DNA sequencing, including the components necessary for the reaction and the steps involved. Topics also include ddNTPs, cycle sequencing, fluorescent dyes and capillary electrophoresis.

We'll also compare Sanger sequencing to other sequencing technologies, such as NGS, to give you a better understanding of how it all works. Throughout the video, we'll take a chronological approach to explore the history of Sanger sequencing, starting with its development in the late 1970s and following its evolution. By the end of the video, you'll deeply understand Sanger sequencing and its role in sequencing the human genome.

So grab a cup of coffee and journey through the fascinating world of Sanger sequencing! Whether you're a student, researcher, or just curious about the science behind DNA sequencing, this video is for you!

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CHAPTERS:
00:00 The 1977 Invention of Sanger Sequencing
00:23 The Basics of How DNA Is Copied
00:36 What are dNTPs & ddNTPs anyway?
01:19 How Does a ddNTP Stop DNA Copying?
01:54 The DNA Naming Convention 5' to 3'
02:43 Which Bases Go Together in Base Pairing?
03:05 Steps of the First Sanger Sequencing Method
06:11 The First DNA Sequencing Instrument, the AB370A.
07:20 The Launch of the Human Genome Project
07:46 How Does Cycle Sequencing Improve Things?
08:59 Automated DNA Separation by Capillary Electrophoresis
09:36 ABI PRISM 310, Modern Sanger Sequencing
10:49 Fluorescent ddNTPs and the Invention of BigDyes
11:33 The Instrument That Sequenced the Genome
13:09 Why Use Sanger Sequencing When There's NGS?

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