How to Screen for CRISPR Knock Out Cells
After successfully using CRISPR to genetically modify your cells, you're ready to move on to the next phase of your project! But how can you ensure that your CRISPR knockout worked as intended? In this video, you'll learn how to confirm a complete knockout and explore three potential strategies to screen your CRISPR knockout clones.
Here are some helpful time stamps for our CRISPR Cas9 Screen It™ Cleavage Detection Kit (Cat. No. G990) video:
0:15 Introduction
2:21 Sanger Sequencing
3:03 Mismatch Cleavage Assay
5:54 In vitro Cas9 Cleavage Detection Assay
7:34 Summary
8:43 Thank You!
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