High-titer Adenovirus and Titration
Recombinant Adenoviruses are wonderful tools used by the scientists for high efficiency, large gene sequence transfers into almost any cell type!
In this video, we'll help you how to perform high-titer viral amplification with adherent or suspension HEK 293 cells, virus purification using iodixanol or Cesium chloride density gradient ultracentrifugation, and virus titration by end-point dilution assay.
Here are some helpful time stamps for our adenovirus purification & titration video:
1:42 High-titer viral amplification with HEK293 cells
4:34 High titer Virus Purification
4:37 Purification by iodixanol gradient ultracentrifugation
6:25 Purification by cesium chloride (CsCl) density gradient ultracentrifugation
7:57 Virus Titration by end-point dilution assay
Watch our video "Adenovirus Production: How to Clone, Package, and Harvest Adenovirus for Your Seed Stocks": • Adenovirus Production: How to Clone, ...
Learn more about the recombinant adenovirus system at our learning resources center: https://info.abmgood.com/adenovirus-s...
Browse our collection of ready-to-use adenoviruses for every human, mouse, and rat gene: https://www.abmgood.com/Adenovirus.html
We also offer ready-to-use CRISPR knockout adenoviruses targeting every human, mouse, or rat gene: https://www.abmgood.com/CRISPR-Knocko...
Need a custom recombinant adenovirus? We can make it for you! Simply tell us your project details and we'll do the gene synthesis, cloning, and adenoviral packaging for you: https://www.abmgood.com/Custom-Recomb...
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