Lentivirus Transduction Protocol: Infecting your target cells
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain:
→ How to perform a lentiviral transduction experiment
→ An overview of the spinoculation and reverse transduction methods
→ How to confirm transgene expression
Lentiviruses are extremely useful gene delivery systems for delivering cDNA, shRNA, and sgRNA to stably express, knockdown, or knockout your gene of interest in your target cells. Once you learn how to use lentiviruses for your own experiment, you'll be able to target not only dividing cells but also post-mitotic, non-dividing cells like neurons.
→ For more information about lentiviruses, access our free learning resources: https://info.abmgood.com/lentivirus-s...
→ Access our full protocol for lentivirus transduction:
https://www.abmgood.com/lentiviral-sy...
Request a free sample of our ViralEntry™ Transduction Enhancer:
→ https://www.abmgood.com/Transduction-...
Watch our video "Multiplicity of Infection (MOI): What is it and how do I calculate it?":
→ • Multiplicity of Infection (MOI): What...
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