A Guide to Next Generation Sequencing Basics and Terminologies | Bioinformatics 101

Опубликовано: 05 Февраль 2024
на канале: Bioinformagician

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In this video, I delve into the intricacies of a standard workflow for next-generation sequencing (NGS). We'll explore essential terminologies and concepts, including sequencing library preparation, multiplexing, sample pooling, as well as the roles of adaptors and barcodes. Furthermore, I provide an overview of different generations of sequencing technologies, spanning from the traditional Sanger sequencing to the advancements seen in Illumina, Ion Torrent, PacBio, and Oxford Nanopore sequencing technologies. I hope you find this video helpful! Leave your thoughts in the comment section below!

▸ Bridge Amplification:
1.    • Overview of Illumina Sequencing by Sy...
2.    • Next Generation Sequencing (Illumina)...

▸ Sequencing by Synthesis (Illumina):
1.    • Intro to Sequencing by Synthesis: Ind...
2.    • Learn about Illumina's Next-Generatio...

▸ What is sequencing depth/depth of coverage?
   • What is sequencing depth? | Bioinform...

▸ Cost to coverage calculator:
1. https://geneglobe.qiagen.com/us/tools...
2. https://support.illumina.com/download...

▸ What is a fastq file?
   • Understanding Bioinformatics File For...

Chapters:
0:00 Intro
0:51 What is Next Generation Sequencing?
1:29 Evolution of sequencing technologies
4:10 A typical NGS workflow
5:01 What is library preparation?
5:47 What is a Flow cell?
7:28 What is multiplexing?
8:59 Index vs barcode
9:17 How many samples to multiplex?
10:39 What is a sequencing library?
11:01 Sequencing run
11:35 Output from sequencing run - fastq

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Website: https://bioinformagician.org/
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