What is Strandedness in RNA-Seq data? | RNA-Seq Stranded Library Construction Methods
In this video I talk about what is strandedness in RNA-Seq data, what additional information do stranded data provide us. In addition, I compare stranded and unstranded RNA-Seq library preparation methods, discuss the terminologies associated with them. Lastly, I also talk about various ways one can determine strandedness in RNA-Seq data.
I hope you find this video helpful! Leave your thoughts in the comment section below!
Parameters for strandedness in various RNA-Seq tools:
https://rnabio.org/module-09-appendix...
Chapters:
0:00 Intro
1:03 Why do we need strand-specific information from RNA-Seq reads?
4:45 A typical RNA-Seq experiment workflow
5:30 Strandedness in transcription
6:39 Library Preparations methods - unstranded and stranded protocols explained
10:08 How are reads stored in a .fastq files for each method?
11:05 Terminologies
11:59 Ways to determine strandedness from RNA-Seq data
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