2) Polymerase Chain Reaction (PCR) - DNA Polymerase
What are the DNA polymerases used in PCR? How do PCR DNA polymerases work?
➜ DNA polymerases are essential for the All forms of PCR including, real time quantitative PCR (qPCR), RT PCR, qRTPCR and reverse transcription, multiplex PCR, touch-down PCR and more. Polymerases are the responsible enzyme for nucleotide assembly and extension thus, essential to the assembly of new DNA molecules. However, there are a number of additional PCR components that also need to be taken into consideration. These included the thermal cycle conditions, fidelity, processivity and buffer components for the polymerase chain reaction. These DNA polymerases are indispensable to cell divisions as they duplicate the genetic information that would be passed to the next generation of daughter cells. Throughout the years, scientists have utilized different variants of DNA polymerases for different applications and prove to be essential for modern molecular biology.
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